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N8267
Sigma
Nitrocellulose membranes for blotting
pore size 0.45 μm, size 15 cm × 15 cm
Description
| Application | Nitrocellulose membranes are the most popular membranes for immunoblotting (Western blotting) of proteins. The basis of protein binding to nitrocellulose is believed to be due to hydrophobic interactions. The 0.45 μm pore size is the most commonly used for proteins greater than 10,000 MW, but for smaller proteins 0.2 μm is recommended to more efficiently trap the proteins.1 |
Properties
| size | 15 cm × 15 cm |
| pore size | 0.45 μm |
Safety
| Personal Protective Equipment | Eyeshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
| Hazard Codes | F |
| Risk Statements | 11 |
| RIDADR | UN3270 4.1/PG 2 |
| WGK Germany | 3 |
References
| Cited Reference | 1. Lin, J.C. and Kasamatsu, H., On the electrotransfer of polypeptides from gels to nitrocellulose membranes. Anal. Biochem. 128, 302-311, (1983) |
| reference | Van Oss, C.J., et al., Mechanism of DNA (Southern) and protein (Western) blotting on cellulose nitrate and other membranes. J. Chromatogr. 391, 53-65, (1987) |
| Gershoni, J.M. and Palade, G.E., Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter. Anal. Biochem. 124, 396-405, (1982) |
